Molecular Biologist (BBBH573291) Genève, Suisse
Kelly Life Sciences is looking for its biotech partner a:
Molecular Biologist
Permanent position in Geneva
Your mission:
You will involve in the project analysis, the design and ordering of optimized nucleotide transgene sequences, the preparation of the corresponding expression vectors, as well as in quality control analyses: verification of a transgene integrated into a cell line by PCR amplification from genomic DNA and amplicon sequencing (Sanger and NGS), mycoplasma testing and assessment of the number of copies integrated in cell lines by ddPCR. The writing of reports as well as the associated documentation are also an important part of your task. The implementation of new methods and technologies, as well as the participation in R&D projects constitute another aspect of this position.
Your responsibilities:
- Sequences optimization including signal peptide screening using bioinformatics tools
- Plasmid cloning and preparation of expression vectors in E. coli
- Purification of DNA fragments
- Genomic DNA extraction and cDNA preparation
- PCR and purification of PCR products for Sanger sequencing
- PCR and preparation of the plate for NGS sequencing
- Sanger and NGS data sequencing analysis
- qPCR (TaqMan and SYBER Green)
- ddPCR (copy number analysis)
- Mycoplasma testing by PCR
- Writing of SOPs and Forms
- Writing of the documentation and analysis reports
- Implementation of new methods / technologies in the molecular biology lab
- Carry out or participate in R&D projects
- Internal project presentation
Your profile:
- Master’s degree or equivalent, no PhD preferred
- NGS data analysis skills
- Capacity to implement new methods and technologies
- Programming for the development of automation bioinformatics tools is a plus
- Fluent in French and good command of English
- 1-5 years’ experience in pharmaceutical or biotechnology
Your contact at Kelly:
Mrs Cristina Alvarino
Consultant Professional Staffing Life Science
Kelly Life Sciences Geneva